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Application of Liquid Chromatography in Pesticide Industry
Application of Liquid Chromatography in Pesticide Industry
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Application of Liquid Chromatography in Pesticide Industry
2-chloro-Nitroacetoacetanilide is an intermediate for the synthesis of pesticides such as quinoxaline, quinoxaline, and quinoxaline. This article uses high-performance liquid chromatography internal standard method to determine the content of p-chloro-o-nitroacetylacetamide. It achieves effective separation of p-chloro-o-nitroacetylacetamide and its impurities, with simple operation, fast results, good reproducibility, and accurate quantification.
1、 Reagents and solutions
Methanol: chromatographically pure, filtered through a 0.45um membrane before use;
Water: Newly distilled secondary distilled water;
Tetrahydrofuran: analytical grade;
Imidacloprid: Impurity peaks without interference;
Standard sample of chloroortho nitroacetylacetamide: known mass fraction>94.6%.
2、 Instrument
LC-3000 high-performance liquid chromatograph; Equipped with a variable wavelength ultraviolet detector;
SW230 column temperature box;
The anti control software for the all Chinese system includes a chromatography workstation;
Chromatography column: C18 (150 mmx4.6mmx5um) stainless steel column;
One hundred thousandth electronic balance;
Ultra pure water system;
Filter: The pore size of the filter membrane is about 0.45um.
3、 Operating conditions for liquid chromatography
Mobile phase: methanol+water=65+35 (v/v); Flow rate: 1.0mL/min; Column temperature: 30 ℃; Detection wavelength: 252nm; Injection volume: 5uL; Retention time: approximately 6min for 2-chloro-Nitroacetoacetanilide and 11.1min for pyrimethanil. The above liquid chromatography operating conditions are typical operating parameters. According to the characteristics of different instruments, appropriate adjustments can be made to the given operating parameters in order to achieve the best results.
High performance liquid chromatograms of 2-chloro-Nitroacetoacetanilide and pyrimethanil.
2-chloro-Nitroacetoacetanilide is an intermediate for the synthesis of pesticides such as quinoxaline, quinoxaline, and quinoxaline. This article uses high-performance liquid chromatography internal standard method to determine the content of p-chloro-o-nitroacetylacetamide. It achieves effective separation of p-chloro-o-nitroacetylacetamide and its impurities, with simple operation, fast results, good reproducibility, and accurate quantification.
1、 Reagents and solutions
Methanol: chromatographically pure, filtered through a 0.45um membrane before use;
Water: Newly distilled secondary distilled water;
Tetrahydrofuran: analytical grade;
Imidacloprid: Impurity peaks without interference;
Standard sample of chloroortho nitroacetylacetamide: known mass fraction>94.6%.
2、 Instrument
LC-3000 high-performance liquid chromatograph; Equipped with a variable wavelength ultraviolet detector;
SW230 column temperature box;
The anti control software for the all Chinese system includes a chromatography workstation;
Chromatography column: C18 (150 mmx4.6mmx5um) stainless steel column;
One hundred thousandth electronic balance;
Ultra pure water system;
Filter: The pore size of the filter membrane is about 0.45um.
3、 Operating conditions for liquid chromatography
Mobile phase: methanol+water=65+35 (v/v); Flow rate: 1.0mL/min; Column temperature: 30 ℃; Detection wavelength: 252nm; Injection volume: 5uL; Retention time: approximately 6min for 2-chloro-Nitroacetoacetanilide and 11.1min for pyrimethanil. The above liquid chromatography operating conditions are typical operating parameters. According to the characteristics of different instruments, appropriate adjustments can be made to the given operating parameters in order to achieve the best results.
High performance liquid chromatograms of 2-chloro-Nitroacetoacetanilide and pyrimethanil.
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