Beckman red blood cell lysate, Beckm red blood cell lysate, Beckman cell lysate, Beckman whole blood red blood cell lysate
Description: Versalyse Lysing Solution, 100 tests

Supplier: Guangzhou Beirui Chromatography Technology Co., Ltd

If you need other types of reagents, please call our company for inquiries.

Red blood cell lysateIt is a relatively mild method for removing red blood cells. Several damaged nucleated cells can effectively remove red blood cells. The tissue cells obtained by red blood cell lysis buffer do not contain red blood cells and can be further used for primary cell culture, cell day balance, separation and extraction of nucleic acids and proteins, etc.

Red blood cell lysisThe principle of

Red blood cells have specific surface antigens on their surface. When the lysis buffer contains enzymes that can attack specific red blood cell surface antigens, it only causes deformation of the red blood cells, expansion, and lysis of biological channels, and does not attack other cells. In addition, red blood cells have their own electronegativity, osmotic fragility, and suspension stability, which are the distinguishing points from other types of cells. Red blood cell lysate utilizes these characteristics to lyse red blood cells.

Red blood cell lysateInstructions for use

1. Add 1 volume of fresh whole blood and 3 volumes of red blood cell lysate. Add 3ml of fresh whole blood to 1mlRed blood cell lysateGently swirl or invert and mix well. two Place on ice for 15 minutes, gently vortex and mix twice during this time. After red blood cell lysis, the solution should be clear and transparent. three Collect cells: Centrifuge at 450 × g for 10 minutes at 4 ℃ to precipitate white blood cells, and carefully discard the supernatant. four Add twice the volume of red blood cell lysate to the white blood cell precipitate, gently swirl to resuspend the white blood cells thoroughly. If the initial blood volume is 1ml, add 2ml of red blood cell lysate. five Centrifuge at 450 × g for 10 minutes at 4 ℃ to precipitate white blood cells, carefully and thoroughly remove the supernatant. six Resuspend cells for subsequent experiments; To extract RNA, start using a solution prepared with DEPC water at this step.

Red blood cell lysateConfiguration method:

Take 100 μ l of anticoagulant whole blood, add 2 ml of application solution 3 #, mix well for 15-20 seconds; add 2 ml of application solution 2 #, mix well for 15-20 seconds; add 4 ml of application solution 1 #, mix well for 15-20 seconds; centrifuge at 300 × g for 2 minutes, discard the supernatant; Collect the precipitate, add PBS buffer, and prepare a white blood cell suspension for later use.

This red blood cell lysis method meets the requirements of white blood cell flow cytometry detection and sorting, and is inexpensive. It is a simple, fast, economical and practical method for white blood cell purification and sorting

Red blood cell lysatePrecautions for Attention

1. This lysis buffer is a sterile product. Please pay attention to aseptic operation when separating cells for cell culture.

2. For your safety and health, please wear lab coats and disposable gloves when operating.

3. 0x storage solution should be stored at 2-8 ° C for no more than six months (six months);

4. Dilute as needed during use, and fresh deionized water should be used to prepare the working solution each time. Dispose of any that cannot be used up on the same day.