FluorCam Open Chlorophyll Fluorescence Imaging System

FluorCamThe open chlorophyll fluorescence imaging system is a highly flexible and widely used instrument for plant physiological and ecological research. The system adopts a modular structure, consisting of four LED light sources, CCD lens, bracket, control unit, and FluorCam imaging analysis software. Two pairs of LED light source boards provide measurement light, photochemical light, and saturation light flicker, with adjustable angles and heights; The height of the CCD lens can be adjusted, and it can be flexibly configured with PSI special high-sensitivity CCD lens or high-resolution lens. It can also be equipped with a fifth light source plate around the lens. The maximum imaging area of the standard configuration (standard version) is13×13 cmThe maximum imaging area of the large version is 20X 20 cm. It can perform chlorophyll fluorescence imaging on both individual leaves and the entire plant, and is widely used in research on plant photosynthetic physiology and ecology, plant stress physiology and susceptibility, stomatal function, plant environment such as soil heavy metal pollution response and biological monitoring, plant resistance, crop breeding, etc.

Application areas:

· Screening of plant photosynthetic characteristics and metabolic disorders

· Detection of biotic and abiotic stress

· Research on Plant Stress Resistance or Susceptibility

· Study on non-uniformity of stomata

· Research on metabolic disorders

· Growth and yield assessment

· Research on the Interaction between Plants and Microorganisms

· Research on the Interaction between Plants and Protozoa

Functional features:

§ Modularization, flexible configuration, free installation and replacement of light source board, free adjustment of light source angle and height, free adjustment of CCD lens height, convenient for the processing and operation of the tested plant

§ Different wavelength LED light source boards can be freely selected, such as selecting a blue light source board for pore function research, selecting a ultraviolet light source board for multi-color fluorescence imaging test measurement, etc

§ The imaging area is large, with the large version having an imaging area of up to 20×20cmIt can perform experimental imaging analysis on entire plants or even multiple plants (such as small plants like Arabidopsis)

§ High sensitivity CCD lens with a time resolution of 50 frames per second, quickly capturing chlorophyll fluorescence transients

§ Automatic repeated imaging measurements can be performed, and an experimental program (Protocols) can be set to automatically cycle imaging measurements. Imaging measurement data is automatically stored in the computer according to the time date (with a timestamp)

§ Equipped with various universal experimental protocols such as Kautsky induction effect and fluorescence quenching analysis, measuring and analyzing over 50 parameters

§ Optional TetraCam color imaging module, with a maximum imaging area of 20×25cm, used for measuring and analyzing leaf or plant morphology

§ Measurement samples include leaves, flowers, fruits, other plant tissues, whole plants, algae, etc

Technical parameters:

§ Imaging area: The standard version has an imaging area of 13 × 13cm, while the large version has an imaging area of 20 × 20cm,Imaging analysis can be performed on plant leaves, plant tissues, algae, mosses, lichens, whole plants or multiple plants, 96 well plates, 384 well plates, etc

§ Measurement parameters:Fo, Fo’, Fs, Fm, Fm’, Fp, FtDn, FtLn, Fv, Fv'/ Fm', Fv/ Fm ,Fv',Ft,ΦPSII, NPQ_Dn, NPQ_Ln, Qp_Dn, Qp_Ln, qN, qL, QY, QY_Ln, Rfd, ETR Waiting for over 50 chlorophyll fluorescence parameters, each parameter can display a 2D fluorescence color image

· Equipped with a complete automatic measurement program (protocol), the automatic measurement program can be freely edited

a) Fv/FmMeasurement parameters include Fo, Fm, Fv, QY, etc

b) KautskyInduction effect: Fluorescence parameters such as Fo, Fp, Fv, Ft_Lss, QY, Rfd, etc

c) Fluorescence quenching analysis: Fo, Fm, Fp, Fs, Fv, QY, Φ_II， More than 50 parameters including NPQ, Qp, Rfd, qL, etc

d) Light response curve LC: Fluorescence parameters such as Fo, Fm, QY, QYLn, ETR, etc

e) PARAbsorption and NDVI (optional)

f) GFPStatic fluorescence imaging measurement (optional)

• high sensitivityTOMI-1CCDSensor:

a) Image resolution: 720 × 560 pixels

b) Time resolution: 50 frames per second

c) A/DConversion resolution: 12 bits (4096 grayscale levels)

d) Pixel size: 8.6 µ m × 8.3 µ m

e) Communication mode: Gigabit Ethernet

§ High resolution TOMI-2 CCD sensor (optional)

a) Line by line scanning CCD

b) Maximum image resolution: 1360 × 1024 pixels

c) Time resolution: up to 20 frames per second at the highest image resolution

d) A/DConversion resolution: 16 bits (65536 grayscale levels)

e) Pixel size: 6.45 µ m × 6.45 µ m

f) Operation mode: 1) Dynamic video mode, used for measuring chlorophyll fluorescence parameters; 2) Snapshot mode, used for measuring fluorescent proteins such as GFP and fluorescent dyes

g) Communication mode: Gigabit Ethernet

§ Light source board: 4 large high-intensity packaged LED light source boards, each light source board is composed of an 8 × 9 LED array, and the effective area of the light source board is the same as the imaging area. The standard version is 13 × 13cm, and the large version is 20 × 20cm

§ Measurement light: Standard 617nm red light, other bands optional, duration adjustable from 10 µ s to 100 µ s

§ Bichromatic light: Standard configuration includes 2 red lights (617nm)+2 white lights, optional 2 red lights (617nm)+2 blue lights (470nm) or other wavelength light source combinations, Actinic1 maximum light intensity300µmol(photons)/m².s， Actinic2 maximum light intensity 2000 µ mol (photons)/m ². s; The maximum photochemical intensity can be upgraded to 3000 µ mol (photons)/m ². s

§ Saturated flicker: maximum light intensity of 4000 µ mol (photons)/m ². s, upgradable to 6000 µ mol (photons)/m ². s

§ PARAbsorption measurement module (optional): Far red light 740nm (FAR), 660nm dual color LED light source board with 7-position filtering wheel and dedicated filter, used for measuring PAR absorption and NDVI

§ GFPMeasurement module (optional): blue photochemical light source, 7-position filtering wheel, and dedicated filter for measuring GFP (green fluorescent protein)

§ 7Position filter wheel (optional): Install different band filters and use a dedicated light source board to measure PAR absorption and steady-state fluorescence such as GFP and YFP

§ UV LED light source board (optional): 365nm or 385nm, 1) measure multi-color fluorescence (see FluorCam multispectral fluorescence imaging system for details); 2) Measure wtGFP (wild-type green fluorescent protein)、 DAPI (4 ', 6-diamidino-2-phenylindole, a fluorescent dye)

§ Blue LED light source board (optional): 470nm, 1) Measurement of EGFP（enhanced green fluorescent protein）； 2）Used for the study of stomatal function

§ greenLEDLight source board (optional): 530nm, measuring YFP (yellow fluorescent protein)

§ Other optional light source boards: Blue (447 nm), Blue (505 nm), Red (627 nm), Deep Red (655 nm), Amber (590 nm), Far Red (740 nm), etc

*measurewtGFP、DAPI、 Fluorescent proteins or dyes such as EGFP and YFP also need to be equipped with a 7-speed filtering wheel, high-resolution CCD, and corresponding filters

§ FluorCamChlorophyll fluorescence imaging analysis software features: Live (real-time testing), Protocols (customized experimental program selection), Pre processing (imaging preprocessing), Result (imaging analysis results) and other functional menus

§ Customized experimental program protocols for customers: can set time (such as measurement light duration, photochemical light duration, measurement time, etc.), light intensity (such as photochemical light intensity of different qualities, saturation light flash intensity, modulated measurement light, etc.), with dedicated experimental program language and scripts. Users can also freely create new experimental programs using the wizard program template in the Protocol menu

§ Automatic measurement and analysis function: An experimental program (Protocol) can be set to automatically perform unmanned cyclic imaging measurement,The number of repetitions and interval time can be customized by the customer,The imaging measurement data is automatically stored in the computer according to the time and date (with timestamp)

§ Snapshot mode: Through snapshot imaging mode,Clear and prominent steady-state and instantaneous fluorescence images of plant samples can be obtained by freely adjusting the light intensity, shutter time, and sensitivity

§ Imaging preprocessing: The program software can automatically identify multiple plant samples or regions, or manually select regions of interest (ROI). The shape of manual selection can be square, circular, any polygon, or sector. The software can automatically measure and analyze the fluorescence kinetics curves and corresponding parameters of each sample and selected area, with no limit on the number of samples or areas (>1000)

§ Data analysis mode: It has "signal calculation and re averaging" mode (arithmetic mean) and "signal average and re calculation" mode. In high signal-to-noise ratio situations, the "signal calculation and re averaging" mode is selected, and in low signal-to-noise ratio situations, the "signal average and re calculation" mode is selected to filter out errors caused by noise

§ Output results: High time resolution fluorescence dynamic image, fluorescence dynamic change video, fluorescence parameter Excel file, histogram, imaging images with different parameters, fluorescence parameter list for different ROIs, etc

§ Give Light System:Static or dynamic (sinus style)

§ CCDDetection range:400–1000nm

§ Spectral response:540nmThe quantum efficiency is highest at 70%, with a 50% decrease at 400nm and 650nm

§ Read out the noise:Below 12eRMS, typical 10e

§ Full trap capacity:Greater than 70000 e (unbound)

§ Bios：Firmware upgradable

§ Communication method:Gigabit Ethernet

§ Supply voltage:90–240V

Typical applications:

Place of Origin:Europe