Sudden changes in heat and cold

Temperature changes can reach several tens of degrees

Consumption of trace sample quantity

Millisecond resolution

principle

This instrument is a new typeThe T-jump instrument changes temperature by mixing solutions with two different initial temperatures. The final temperature value is calculated based on the initial temperature and mixing ratio of the two solutions.

The device includes three Peltier elements, which control the temperature changes of two initial solutions and the colorimetric cell after mixing.The mT jump accessory needs to be installed on the Bio Logic basic flow stop device.

Full control of the rapid mixing mutation system can be achieved through Bio Kin software: through the software, users can define the reaction mixing ratio and temperature mutation span. The Peltier element will automatically adjust the temperature. Automatic accumulation injection will optimize the signal-to-noise ratio.

The mT jump system can use a standard colorimetric cell and is used for various forms of spectroscopic measurements such as circular dichroism, UV absorption, fluorescence anisotropy, etc.

Supplement to the traditional T-jump system

The traditional T-jump device is used to record the rapid thermal effects of reactions. Usually achieved using fast thermoelectric current pulses or applying more near-infrared pulses to the sample. The time resolution can reach several microseconds, but due to temperature dissipation, dynamic data cannot be recorded for more than a few milliseconds, which limits certain experimental applications. Most temperature mutation devices have a mutation range limited to 5 ℃ and cannot achieve cold mutation yet.

The mT jump rapid mixing temperature mutation system developed by Bio Logic is based on dwell technology and has millisecond level resolution. The magnitude of the mutation is completely determined by the user (up to a mutation span of 40 ℃), and both hot and cold bidirectional mutations can be achieved. Cold and hot bidirectional mutations have expanded the field of applied research, such as refolding studies, which do not require the addition of denaturators to proteins for protein folding and refolding experiments. In addition, traditional T-jump systems can only be used for absorption and fluorescence measurements, while mT jump systems are compatible with all measurement modes, including circular dichroism, Fourier transform infrared spectroscopy, fluorescence anisotropy spectroscopy scanning, and so on.

Specification

Temperature Control System Description

USB connection, fully software controlled operation

Temperature range:+5 ℃ to+90 ℃

Temperature accuracy: 0.01 ℃

Maximum temperature mutation:+/-40 ℃

Temperature stability in the colorimetric cell after a sudden temperature change: less than 1% change every 30 seconds

Includes temperature probe

Temperature stability of syringe 1 and syringe 2 in steady state: less than+/-0.2 (after 15 minutes of equilibrium at 80 ℃)

Mixed description

Can be connected to all Bio Logic kinetic flow stop devices

Mixing ratio from 1/1 to 1/4

Sample size for each injection: 45 μ l to 220 μ l

Dead time: determined by colorimetric pool, stop flow mode, and mixing ratio (0.7ms, using SFM-2000, FC-08 colorimetric pool)

Can be connected to other devices such as MOS-200, MOS-450/AF-CD, secondary array tube detector, etc

Can be paired with Fourier transform infrared colorimetric cell.