PlantScreen Plant Phenotype Imaging Analysis System (Plant Automatic Transfer Version)

PlantScreenThe plant phenotype imaging system is developed and produced by PSI company in the Czech Republic,It integrates advanced technologies such as LED plant intelligent cultivation, automated control system, chlorophyll fluorescence imaging measurement and analysis, plant thermal imaging analysis, plant near-infrared imaging analysis, plant hyperspectral analysis, automatic barcode recognition management, RGB true color 3D imaging, automatic weighing and watering system, etc,To achieve comprehensive physiological, ecological, and morphological imaging analysis of a large number of plant samples in an optimized manner, from Arabidopsis thaliana, corn to various other plants, for high-throughput plant phenotype imaging analysis, plant stress response imaging analysis, plant growth analysis, ecological toxicology research, trait identification, and plant physiological and ecological analysis research. Plant automatic teleportation versionPlantScreenThe system is mainly suitable for plant samples with a height of 0-40cm.

PlantScreenThe system includes the following imaging analysis functions:

1.Chlorophyll fluorescence imaging analysis: single imaging area of 35x35cm, imaging measurement parameters include Fo, Fm, Fv, Fo’, Fm’, Fv’, Ft, Fv/Fm, Fv’/Fm’, Phi_PSII, NPQ, qN, qP, Rfd Waiting for dozens of chlorophyll fluorescence parameters

2.RGBImaging analysis: Imaging measurement parameters include:

1)Leaf Area: Useful for monitoring growth rate）

2)Plant solidity/compactness. Ratio between the area covered by the plant’s convex hull and the area covered by the actual plant）

3)Leaf circumference: Particularly useful for the basic leaf shape and width evaluation (combined with leaf area)）

4)Eccentricity: Plant shape estimation, scalar number, eccentricity of the ellipse with same second moments as the plant (0...circle, 1...line segment)）

5)Roundness of leaves: Based on evaluating the ratio between leaf area and perimeter. Gives information about leaf roundness）

6)Medium Leaf Width Index: Leaf area proportional to the plant skeleton (i.e. reduction of the leaf to line segment)）

7)Slenderness of Leaves (SOL)

8)Plant Circle Diameter. Diameter of a circle with the same area as the plant）

9)Convex Hull Area. Useful for compactness evaluation）

10)Plant Centroid. Center of the plant mass position (particularly useful for the eccentricity evaluation)）

11)Internodal distances

12)Growth Height

13)Maximum Height and Width of Plant in 3 Dimensions

14)Relative growth rate

15)Leaf Angle

16)Leaf Number at Nodes

17)Other parameters such as color segmentation for plant fitness evaluation, greening index and others

3.Hyperspectral imaging analysis (optional), capable of imaging and analyzing the following parameters:

1)Normalized Difference Vegetation Index (NDVI)

2)The Simple Ratio Index,Equation: SR = RNIR / RRED）

3)Improved Chlorophyll Absorption in Reflectance Index (MCARI1),
Equation: MCARI1 = 1.2 * [2.5 * (R790- R670) - 1.3 * (R790- R550)]）

4)Optimized Soil Adjusted Vegetation Index (OSAVI)
, Equation: OSAVI = (1 + 0.16) * (R790- R670) / (R790- R670 + 0.16)）

5)Greenness Index (G),Equation: G = R554 / R677）

6)Improved Chlorophyll Absorption in Reflectance Index (MCARI),
Equation: MCARI = [(R700- R670) - 0.2 * (R700- R550)] * (R700/ R670)）

7)Transformed CAR Index (TCARI), Equation: TSARI = 3 * [(R700- R670) - 0.2 * (R700- R550) * (R700/ R670)]）

8)Triangular Vegetation Index (TVI), Equation: TVI = 0.5 * [120 * (R750- R550) - 200 * (R670- R550)]）

9)ZMIZarco Tejada&Miller Index (ZMI),Equation: ZMI = R750 / R710）

10)Simple Ratio Pigment Index (SRPI),Equation: SRPI = R430 / R680）

11)Normalized Phaeophyteinization Index (NPQI),Equation: NPQI = (R415- R435) / (R415+ R435)）

12)Photochemical Reflectance Index (PRI),Equation: PRI = (R531- R570) / (R531+ R570)）

13)Normalized Pigment Chlorophyll Index (NPCI), NPCI = (R680- R430) / (R680+ R430)）

14)CarterCarter Indices
, Equation: Ctr1 = R695 / R420; Ctr2 = R695 / R760）

15)LichtenthalerLichtenhaler Indicators, Equation: Lic1 = (R790 - R680) / (R790 + R680); Lic2 = R440 / R690）

16)SIPIStructure Intensive Pigment Index (SIPI),Equation: SIPI = (R790- R450) / (R790+ R650)）

17)Gitelson-MerzlyakGitelson and Merzlyak Indices, Equation: GM1 = R750/ R550; GM2 = R750/ R700）

4.Thermal imaging analysis (optional): used for imaging analysis of the two-dimensional heat distribution of plants under light radiation conditions. Good heat dissipation can enable plants to tolerate long periods of high light radiation or low water conditions (drought)

5.Near infrared imaging analysis (optional): used to observe and analyze the water status of plants and their distribution variation among different tissues. Plants in good irrigation conditions exhibit high absorption of near-infrared spectra, while plants in drought conditions exhibit high reflectivity of near-infrared spectra. Through analysis software, the entire process from drought stress to re irrigation can be monitored and analyzed dynamically, as well as the plant's response to drought stress and water use efficiency, and false color images can be formed, which can be correlated with the plant's morphological index and chlorophyll fluorescence index for analysis and research.

System configuration and working principle:

The entire system consists of an automated plant transport system, a light adaptation room, RGB imaging FluorCamIt consists of chlorophyll fluorescence imaging, hyperspectral imaging, plant thermal imaging, plant near-infrared imaging, automatic watering, fertilization and weighing system, plant identification system, etc. Plants in the light adapted room can be transported to the imaging room for imaging analysis by a conveyor belt. The entire system can be customized according to the actual needs of users, highly meeting their actual needs.

Technical indicators:

1.Automatic loading and unloading of plant samples，Identify and track samples through barcode or RFID tags

2.Light adaptation room: used for light adaptation or plant cultivation, LED light sourceThe light intensity reaches 1000 μ mol/m².s， No thermal effect, adjustable intensity from 0-100%, can preset light cycle changes through experimental programs, can be equipped with universal or specialized models such as rice growth observation rooms, and can also be equipped with 3D scanning imaging analysis functions (including XYZ 3D scanning imaging system and software)

3.Standard tray holder 35 × 30cm, used for placing potted plants or trays that can hold multiple small flower pots. Each tray can hold 20 standard plant culture pots (58mm × 58mm × 95mm), and can be customized with a maximum pot size of L35cm × W28cm

4.The automatic transmission system forms a circular transmission channel from the light adaptation room to the imaging room. The transmission belt adopts a three-phase asynchronous motor with a gearbox, with a power of 200-1000W, a transmission bandwidth of 320mm, a load force of 130kg, and a speed of 9m/min

5.Central processing unit of mobile control system: CJ2M-CPU33; Digital I/O: maximum 2560 points; PLC communication: via Ethernet 100Mb/s high-end PC; OMRON MECHATROLINK-II maximum 16 axis precise positioning

6.Plant imaging measurement room: 150cm (length) x 150cm (width) x 220cm (height), isolated from ambient light (light isolated), quickly and automatically opens and closes the door, with an opening and closing cycle of less than 3 seconds, and transported into the mouth with a light curtain sensing system, barcode recognizer, and RFID reader

7.Standard imaging room imaging unit height adjustable from 0-50cm, optional 100cm or higher range height for studying tall plants, standard focal length range 22-27cm

8.RFIDReader recognition distance: 2-20cm; Communication: RS485; The barcode recognizer can read 1D, 2D, and QR codes, with an LED light source for easy identification in low light conditions and RS485 communication

9.Customer customized F3EM2 light curtain system, precise measurement of plant height and width for automatic precise positioning of the camera after entering the imaging measurement room, measurement range of 150cm, resolution of 5mm; standard laser height measurement 0-50cm, accuracy of 5mm

10. Chlorophyll fluorescence imaging: including light isolation imaging room, automatic opening and closing door, conveyor belt、 PLC controlled automatic up and down movement focusing system, large 73 × 73cm LED light source board, 7-bit filtering wheel, etc., single imaging area of 35 × 35cm, measurement light orange 620nm, orange and white dual wavelength photochemical light, saturated light flash white, maximum light intensity 3600 μ mol.m-2. s-1, lens resolution 1360 × 1024 pixels

11.Automatic irrigation and weighing,It can irrigate and weigh 5 plant planting pots simultaneously with an accuracy of ± 1g. After weighing, precise watering can be carried out, and the watering process (regime) or drought stress state can be preset through an experimental protocol. A nutrient supply system can also be selected to quantitatively supply plant nutrients (such as nitrogen fertilizer, etc.) with watering; Automatic zero calibration before weighing, and automatic recalibration can also be performed using known weight items (such as weights); Protection level: IP66

12. The customer customized weighing system consists of 4 weighing units, with a safe bearing limit of 150% Ln; temperature compensation of -10-40 ° C, standard measurement range of 7kg, optional 10kg, 15kg or 20kg; standard weighing system is used for precise weighing of standard plant culture pots, with a maximum load capacity of 300g, resolution of 1g, accuracy of 0.5g, and measurement parameters including actual weight, watering amount, etc

13. RGBImaging: Top and side 3D imaging (3 cameras), each camera has its own independent control panel to set exposure time, gain, white balance, etc. The snapshot button on the control panel can be used to take real-time photos and display resolution and other information. It can also be automatically imaged in automatic mode and stored in the database. The imaging time for each scan is less than 10 seconds, and 3D modeling function is optional

14.RGBThe imaging system includes an imaging room (optical isolation), conveyor belt and position sensor, 3 cameras, light source and imaging analysis software, with a standard imaging area of 3535cm x 35cm, focal length range 22-27cm, LED cool white light source (does not produce thermal effects on plants)

15.Standard USB Ethernet camera with 2592 effective pixels1944, bit resolution of 12 bits, optical quantum efficiency: blue peak 465nm, green peak 540nm, red peak 610nm; 28mm optical lens, aperture 43.2mm, aperture range 2.8-F16

16. NIRNear infrared imaging unit: capable of capturing images in the 1450-1600nm water absorption band to reflect plant moisture status. It exhibits high NIR absorption values under abundant water supply and high NIR reflection under drought stress. NIR false color imaging can be used to reflect and analyze plant moisture status through software

17.The hyperspectral imaging unit includes an optical isolation imaging measurement room, an automatic opening and closing door, a conveyor belt, a PLC controlled automatic moving focusing lens including SWIR and VNIR lenses, a light source, an imaging analysis system, etc. The VNIR lens has a wavelength range of 380nm-1000nm, an aperture of F/0.2, a gap width of 25 μ m, a gap length of 18mm, and a frame rate of 12-236 fps; SWIR lens band 900-2500nm, aperture F/0.2, gap width 25 μ m, gap length 18mm, frame rate 60 or 100 fps, imaging area 35 × 35cm

18.Users can choose SWIR imaging, VNIR imaging, or two lens full band imaging through the experimental program, with each lens having an imaging time of 15 seconds

19.Thermal imaging unit: resolution 640 × 480 pixels, temperature range -20-120 ° C, sensitivity NETD<0.05 ° C @ 30 ° C/50mK, accuracy ± 2 ° C, standard imaging area up to 35 × 35cm, focal length range 40-50cm, white light source, maximum light intensity 500 μ mol.m-2. s-1, 0-100% adjustable

20.FS-WILarge plant growth chamber

ØLight source: cool white LED (6500K)+far red LED (735nm), other light sources such as RGB tri color light source board can be customized and controlled from 0-100%, dedicated light source cooling airflow channel, programmable simulation of natural light environment changes such as day night cycle changes, sunrise and sunset, as well as various other arbitrary changes

ØMaximum homogeneous light intensity: 1000 µ mol (photons)/m ². s, customizable for higher light intensity

ØTemperature control range: 10 ℃ -40 ℃ (control effect depends on light intensity and ambient temperature, with a maximum room temperature of 30 ℃), customizable for a larger temperature control range, programmable to simulate temperature changes in nature such as day night cycles, sunrise and sunset, as well as various other arbitrary changes

ØHumidity control range: 40-80% ± 7% (control effect depends on light intensity), programmable simulation of natural humidity changes such as day night cycle, sunrise and sunset, as well as various other arbitrary changes

21.System Control and Data Collection Analysis System:

ØUser friendly graphical interface

ØUser defined, editable automatic measurement programs (protocols)

ØMySQLA database management system that can handle large databases with millions of records, supports multiple storage engines, and automatically stores relevant data in different tables in the database

ØPlant code registration function: including storing plant identification codes, tray identification codes, etc. in the database, automatically extracting and reading barcodes or RFID tags during measurement

ØTouch screen operation interface, online display of plant tray quantity, light intensity, analysis and measurement status and results, etc., easily and completely control all mechanical components and imaging workstations through software

ØAll measurements can be performed using the default program, or custom workflows can be created through development tools, or manually operated to turn on or off LED light sources, RGB scanning imaging, chlorophyll fluorescence imaging, weighing and watering, etc

ØThe experimental protocols have start, end, and pause keys

ØAutomatically control the movement of plant samples and activation of a single imaging station according to experimental requirements

ØCan provide RGB digital growth analysis with 3 camera angles, including threshold analysis and color analysis

ØFor chlorophyll fluorescence imaging images, the software can perform batch quenching parameter analysis, including the average of user interested regions and pixel values on the background removed image. The analysis data is stored in the database in the form of raw images and analysis data.

ØFor FIR thermal imaging images, 16 bit maps can be directly exported to MATLAB or generate false color images of temperature distribution through software.

Place of Origin:Europe