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Purification of high pressure protein in 10-200ml min
Purification of high pressure protein in 10-200ml min
Product details
PPS-10-200 High Pressure Protein Purification System: 15-35Mp (Application level: small experimental type, semi preparation type, small preparation type)
System Characteristics
Modular design
Convenient for users to choose and match the required equipment, and with a small footprint. All valve and other pipeline joints are located on the right side, and a reasonable pipeline layout greatly reduces dead volume in the pipeline. Connecting the instrument backend to a fixed column can form a whole, making it convenient for users to choose and match.
infusion system
Dual plunger parallel mode, series mode, floating plunger design, online real-time cleaning function, maintenance free.
system detection
The online 190nm-700nm UV detector (or other online detectors) uses imported deuterium and tungsten lamp light sources, ensuring reliable quality.
PH conductivity
The spectral lines of online pH conductivity detection facilitate users to grasp experimental data and conditions in real time, greatly shortening the experimental process.
Component collection
Robot arm mode, high-precision positioning, powerful automatic collection capability, supports full collection, and peak collection (threshold, slope).
software
The automatic buffer preparation function facilitates quick exploration of purification processes, and the corresponding parameters are programmed according to on-site process requirements. Real time monitoring and control of flow paths; The same screen displays UV, gradient spectral lines, and collection tube numbers. Automatic software control improves the purity and repeatability of collection, enabling the system to obtain more separation information of samples and impurities in a timely manner, making the tedious chromatographic purification process easy and convenient.
technical specifications
| Light source s | Imported deuterium lamps and tungsten lamps |
| wavelength range | 190nm-700nm continuous online wavelength setting, no need to change the lamp, can detect 2 wavelengths simultaneously. |
| Wavelength online scanning | Full range scanning from 190nm to 700nm |
| Wavelength accuracy | ±1nm |
| Wavelength reproducibility: | 0.2nm、 |
| baseline noise | 0-5AU ±0.5×10-5AU |
| Range of absorbance | -5AU--5AULinear: ± 2%, between 0-2 AU |
| pump head | Material: Stainless steel pump head (ceramic and PEEK optional), imported ruby ball one-way valve, high precision, long service life. |
| flow rate range | 0.01-99.99ml/min, 0.1-50ml/min, 0.1-100ml/min, 200ml/min (flow rate can be customized) |
| Flow velocity accuracy | ±1% |
| Traffic reproducibility | ±1% |
| Maximum pressure | 35MP、25MP、20MP、15MP |
| gradient type | Linear, isomorphic, and adjustable gradient online |
| Static Mixer | Online solution mixing, using countless ceramic microspheres to ensure uniformity during gradient mixing, with the function of online filtration to protect the chromatography column. The mixer has 4 tubes, with one tube being 2ml and two tubes being 4m Gradient mixing accuracy: ± 0.5%, gradient mixing accuracy: 0.3% RSD does not vary with backpressure Maximum dead volume: 8ml |
| Online conductivity meter Online pH meter |
Conductivity measurement range: 1us-99.99ms; Equipped with temperature detector: temperature range: 0-99 ° C; Temperature accuracy: ± 1.5 ° C between 4 ° C and 45 ° C, accurately reflecting temperature changes. PH measurement range: 0-14.00pH |
| Sample size and mode | Set the sample size according to the user's process requirements, （Sample can be loaded onto a quantitative tube, 200ul-5ml, or add a conversion valve to the sample pump) |
| Component collection or Valve channel collection |
Robot arm mode: high-precision positioning Peak Collection: Automatically collect based on volume, time, threshold, and slope. Collection modes: Normal mode, sequential collection, and cyclic collection, making collection more intelligent and user-friendly Adopting imported high-precision three-way valves while utilizing bottle cutting technology, the waste liquid has an independent channel, and there is no dripping or leakage during the bottle switching process The supporting software can collect multiple wavelength signals in real time, and the collected signals can be selected arbitrarily Capacity: 120 test tubes (height 90-180mm, height x 13-15mm diameter); 80 test tubes (height 90-180mm, height x 18mm diameter); Valve collection: 7 channels for collecting samples and 1 channel for discharging waste liquid. |
| Biological freezer | Temperature range: 1-10 ℃, power: 850w, size: 1800 * 800 * 1960 (external dimensions) 1680 * 630 * 1400 (internal dimensions) |
| power supply | 220VAC/110VAC |
| Power frequency | 50Hz |
| operation temperature | 1-40℃ |
| Control Interface | RS-232C(USB)， Using a PC based on Windows XP or Windows 7 The software control platform can display UV, pH, conductivity, gradient spectra, and display the collected test tube number. |
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