1、 Introduction to Method and Principle

Standard basis: National "Hygienic Standards for Drinking Water in the People's Republic of China"（GB 5749-2006）Microbial indicators for Giardia and Cryptosporidium have been added to the requirements<1individual/10LThe testing method used for this product is specified by the national standard“Two worms”IFADetection method, sampling and washingIMSAll systems comply with national standards and the United StatesEPA1623The standard method.

The specific steps are as follows: filter sampling→Wash and concentrate→ IMSMagnetic separation→ IFAdye→Microscopic Examination

2、 Description of main characteristics and technical progressiveness

（1） The standardization, rationality, and applicability of the two insect system design

1、 AmericaUniBestcompanyCompactType Two Insect DetectorIFAThe staining system complies with and exceeds the Chinese national standard.

2、 AmericaUniBestcompanyCompactType Two Insect DetectorIMSThe immune magnetic separation system adopts the national standardGB/T 5750.12-2006method.

3、 AmericaUniBestcompanyCompactThe filtration system of the Type 2 insect detector adopts disposable filter bags that comply with international technical specifications.

（2） Reliability and stability of the two insect system

1、 Recovery rate of filtering steps ≥100%

2、 Recovery rate of washing steps≥80%

3、 IMSRecovery rate of immune magnetic separation step≥90%

4、 Total recovery rate (filtration, washing, magnetic bead separation, staining)≥ 35%

5、 Relative standard deviation of test results≤10%

6、 Microscopic examination rate: Giardia cysts100%Detected, Cryptosporidium oocysts100%detection

7、 Detect high turbidity water samples that can be processed: up to 100（NTU）

8、 The reagent kit is equipped with pre made positive control slides to ensure the effective activity of the kit; Completely avoid false positives and ensure the accuracy of testing.

9、 Long shelf life: United StatesUniBestYoubaida CompanyClearScanSeries Two InsectsIFAStaining reagents have a long shelf life 24 Months is a longer shelf life and more stable performance in the industryIFAReagents.

（3） Progressiveness, accuracy and economy of the two insect system

1、 Specially labeled monoclonal antibodies: including anti Cryptosporidium antibodies and anti Giardia monoclonal antibodies, fluorescein isothiocyanate（FITC）It is a special immunoglobulin with specific effectsMPentamer, composed of5 individualFITCSimultaneously labeling an antibody at the binding site has a significant bright fluorescence effect, and this antibody is a staining reagent recommended by the national standard.

2、 Provide special effect color fixing solution: The color fixing solution makes the color more stable, and even after a few days, it still does not fade. The microscopic inspection effect is still clear.

3、 AmericaUniBestYoubaida CompanyClearScanseriesIFAThe staining reagent has been added with a specialized counterstaining solution, which can make the focusing more accurate and the counting clearer and more precise.

4、 Provide positive control slides: reagent kit（25 The test quantity includes a pre made glass slide for real-time self inspection, making testing more convenient and time-saving.

5、 The sampling and filtering system adopts the United StatesEPA1623The standard recommended disposable filter bag completely eliminates sample cross contamination; It can completely replace disposable or reusable filtering equipment from other brands without changing all experimental instruments, reagents, and equipment.

6、 AmericaUniBestcompanyClearScanThe series of two insect detection systems have significant advantages in daily detection and operation costs compared to similar products in the market.

IIISystem Overview

（1） Sampling, washing, and concentration system

Adopting standards that comply with Chinese national standards and the United StatesEPAThe required bag filter is used to filter water samples, collecting spore sacs, egg sacs, and other substances on the filter. This filter can be used for laboratory and field water sample filtration. The filter bag uses two insect specific washing buffer to shake and wash off the two insects intercepted on the filter bag, and finally collects them by centrifugation.

(II)Immunomagnetic Separation System（IMS）

IMSProcess using the United StatesUniBestUbaida Company'sClearScanThe series of two insect specific immune magnetic separation kits use magnetic beads with specific antibodies to bind with specific antigens of Giardia and Cryptosporidium in the sample, and efficiently and selectively separate them in a magnetic field. This step ensures the specificity of detection.

（3） TheIFAIndirect immunofluorescence staining system

Using the United StatesUniBestUbaida Company'sCellabsType detection kit for diagnosing Giardia and Cryptosporidium (containing specific immunoglobulin)MpentamerFITCFluorescent labeling of two worms (antigens) in water samples using monoclonal antibodies, while utilizing nuclear staining agentsDAPI（4，6-Diamino group-2-Phenylindole) and double stranded molecules in the nucleus of the cellDNACombining fluorescence emission to eliminate false positive reactions, thereby improving the accuracy of microscopic examination.

（4） Microscopic inspection system

Use a two insect specific fluorescence microscope to detect the colored sample, observe the sample, and observe the observed object that conforms to the size, shape, and fluorescence characteristics of the sporangia and egg sacs according to the national standardGB/T 5750.12-2006Qualitative and quantitative analysis of the characteristics of flagellate cysts and cryptosporidium oocysts in Zhongjiadi (see last page).

5、 Remarks

According to the national standardGB 5750 12-2006According to the "Standard Inspection Method for Drinking Water",“Two worms”The detection system can be roughly divided into the following five steps: filtering and sampling→Wash and concentrate→ IMSMagnetic separation→ IFAdye→Microscopic examination, where filtering sampling is only“Two worms”A non core step in the detection process, the model of the filter mentioned in the experimental method cannot represent the essence of the sampling method for two insect detection out of context, nor can it represent the detection method for the entire process of two insect detection in a biased manner; The filter sampler is only a tool for collecting samples, not a key technology for two insect detection. With the advancement of two insect sampling technology, as long as the two insect sampler used achieves the purpose of sample collection, it is sufficient; If only one model of filter sampler is used to refer to the experimental method of national or even international standards, it will confuse the two insect detection methods and the original essence of national standards.

AmericaUniBestUbaida Group's“Two worms”The detection system adopts a one-time filter bag filtration sampling method, and its performance indicators not only meet but also exceed the national standard (the national standard requires the pore size of the filter bag to be1μmOur aperture is0.45μmWith a smaller aperture, it can ensure the safety of both insects100%Completely intercept,).

“Two worms”The core steps of detection areIFAIndirect immunofluorescence staining, United StatesUniBestYoubaida Group“Two worms”Staining reagents are the staining reagents specified in the core steps of the national standard.