second-handAlltechELSD 2000ES, Alltech 3300 Evaporative Luminescence Detector

Alltech ELSD 2000ES,Alltech 2000,800,3300,ELS Detector

The Alltech 2000ES Evaporative Light Scattering Detector 2000 Enhanced Sensitivity is designed for use in high-performance liquid chromatography systems to analyze any compound with lower volatility than the mobile phase. The application scope of ELSD includes carbohydrates, drugs, lipids, triglycerides, undifferentiated fatty acids and amino acids, polymers, surfactants, nutritional supplements, and combination molecular libraries. The evaporative light scattering detector eliminates common issues found in other HPLC detectors. Differential detection is complicated by the interference of solvent front peaks, and due to its sensitivity to temperature, the baseline is very unstable and incompatible with gradient elution. In addition, the response of differential detectors is not as sensitive as ELSD. Low wavelength ultraviolet detectors are plagued by baseline drift under rapidly changing gradient conditions and require the analyzed compound to have chromophores. ELSD is not subject to these restrictions. Unlike these detectors, ELSD can obtain stable baselines in the presence of multiple solvent gradients, resulting in good resolution and fast separation speed. In addition, because the response of ELSD does not depend on the optical properties of the sample, ELSD detection does not require the sample to have chromophores or fluorescent groups.

The Alltech 2000ES evaporative light scattering detector is a newly launched 2000 enhanced ELSD by American company Outec

The Alltech 2000ES evaporative light scattering detector inherits the advantages of the 2000 ELSD: dual mode with split current and no split current; Four zone temperature control completely eliminates the interference of environmental changes on the analysis results; Unique self checking function facilitates troubleshooting; Large screen LCD microcomputer control facilitates human-machine dialogue; The 90 degree detection angle eliminates the interference of false signals. At the same time, improvements have been made to the optical detection and circuit parts of ELSD. The 2000ES light cell and light trap are coated with inert and corrosion-resistant PTFE plastic, and the light trap tail blowing is added to reduce maintenance requirements, improve system durability, and reduce background noise. Compared to ELSD 2000, ELSD 2000ES has increased sensitivity by more than an order of magnitude!

The evaporative light scattering detector has better universality than the ultraviolet absorption detector and higher sensitivity than the differential (refractive) detector. Compared to mass spectrometry, ELSD is more affordable, requires lower maintenance, and is suitable for developing quantitative analysis techniques. Therefore, ELSD has been widely applied in the field of analytical technology since its inception.

In addition, differential detection is complicated by the interference of solvent front peaks, and due to its extreme sensitivity to temperature, the baseline is very unstable and incompatible with gradient elution; Low wavelength ultraviolet detectors are plagued by baseline drift under rapidly changing gradient conditions and require the analyzed compound to have chromophores. ELSD is not subject to these limitations, as it can obtain stable baselines in the presence of multiple solvent gradients, resulting in better resolution and faster separation speed. At the same time, the response of ELSD does not depend on the optical properties of the sample, so ELSD detection does not require the sample to have chromophores or fluorescent groups, and more accurately represents the mass composition of the sample.

Alltech ELSD 2000ES Technical Specifications

Operation mode:

Split or non split dual-mode, freely choose according to different needs during use: for relatively low boiling point substances, choosing split mode can reduce drift tube temperature, reduce sample volatilization, and thus improve sensitivity. For relatively high boiling point substances, the non split mode is selected to allow the sample to fully enter the detection element after passing through the chromatographic column, thereby achieving high sensitivity.

Light source:

Laser diode with light correction system, 650nm, high output 30mw, compliant with FCC safety standard IIIA. The light source has a lifespan of up to 30000 hours and maintains a constant light intensity throughout its effective lifespan. The use of Brewster angle hydrazine effectively reduces background noise

Detection angle:

The photodiode detects scattered light from a 90 degree angle. Avoiding the drawbacks of some manufacturers' detectors receiving refracted or diffracted light when detecting from angles greater than 90 degrees, and also avoiding the possibility of false signals caused by some manufacturers' detectors receiving reflected light when detecting from angles less than 90 degrees.

Detection limit:

Use a standard column with a signal-to-noise ratio of 5, calculated as 2ng of hydrocortisone; use a narrow diameter column with a signal-to-noise ratio of 5, calculated as 0.5ng of hydrocortisone; use a micro diameter column with a signal-to-noise ratio of 5, calculated as 0.1ng of hydrocortisone.

Temperature range:

The four areas of atomizer, drift tube, detection cell and discharge outlet are independently controlled to maintain a constant temperature difference between the spray outlet and the discharge outlet and a constant settling time of the sample in the drift tube. Drift tube with room temperature up to 120 ℃, built-in temperature compensation system for more precise temperature control.

Atomized gas:

0-5L/min adjustable, built-in digital flowmeter control, not affected by pressure changes.

Spray pressure 60-80PSIG

Tail blowing function with SFC interface

Automatic zeroing of signal output

Gas switch control automatic

Error correction system: PC intelligent control and correction of gas flow rate, gas pressure, and temperature.

Automatic alarm for safety function

Atomization temperature control with or without diversion, full temperature control throughout the process

Drift tube construction all made of high-quality stainless steel structure (excluding fragile glass and corrosive components)

The instrument itself can store 10 methods for selecting and displaying operating parameters, and the initialization of the instrument is fast and reliable. LCD chart display configuration

Choose between using a numeric keypad for control or a PC for control.

Analog output with dual options of 0-1V or 0-10MV, 5 levels of attenuation, adjustable.

The sample range can detect semi volatile and non-volatile compounds

Power supply 120/240V, 50/60HZ

Size 23.0 "high X12.5" wide X21.6 "deep (58.4cm high X31.8cm wide X54.8cm deep)

Weight 35 pounds (16KG)